Abstract
Result of the investigation on distribution of Gnathostoma spinigerum Owen, 1836 on the basion of Tsuboi- and Shirakawa-River flowing through Kumamoto-City:
1) Basions of a branch of Tsuboi-River and Iseri-River were found to be distributing places of Gnathastoma spinigerum Owen, 1836 from the investigation of Ophicephalus argus.
2) As to second intermediate host of Gnathostoma spinigerurn Owen, 1836 distributed on the low Tsuboi- and Shirakawa-River, Ophicephalus argus and Parasilurus asotus were reported.
3) Parasite parts were found in the muscle of Ophicephalus argus and Parasilurus asotus. The latter was found newly as the host of Gnathostoma spinigerum Owen, 1836.
4) As ta Cyprinus auratus, Anguilla japonica, Misgurnus anguilli caudatus, Odontobutis obscura, Rana nigromaculata nigromaculata, R. rugosa, Eriocheir japonicas, Sesarma haematocheir, S. intermedia, S. dehaani, Cambarus clarkii on the basions of Tsuboi and Shirakawa-River and C. auratus, Parasilurus asotus, R. nigromaculata nigromaculata, R. rugosa on the basion of Iseri-River were not found.
