Abstract
1) Experimentally, gastric lymphvessels and its lymphnodes were well stained by the coloring matters (3% directsky blue) which injected in gastric submucosa.
As a result, it was recognized that the anomalous ref lux of lymphf low appeared because of artificial ligations of lymphvessels.
2) In man, the coloring matters were injected at the submucosa near to the tumor through the fiber-gatroscope before the laparatomy, but the infiltrated area of gastric wall and metastatic lymphnodes were not stained.
3) Our method of vital staining is very convenient at the time of radical operation of gastric cancer.