Abstract
Enzyme examination of serum GOT and GPT is widely performed in clinics. The standard method for the assay of these enzymes was first proposed by Wroblewski and Karmen, who measured the oxidation velocity of DPNH by coupling the transaminases with DPNH-oxidizing enzymes. This method requires too much time for the examination. The alternate of this method is now rather common, in which 2, 4-dinitrophenyl hydrazine is used as a trapping agent and the hydrazones of both substrate and product are measured colorimetrically. Although the latter method is more advantageous in carrying out with many samples, measurement error is apt to be caused.
We have tried to assay transaminases essentially according to Wroblewski and Karmen, but using a recorder to spare working time, and obtained a good result. This paper deals with several problems when one uses a recorder for routine examinations of transaminases: 1) how to use a recorder, 2) sources of error, 3) relation to the hydrazine method.
