Abstract
This report dealt with the distribution of the DNA content in cells obtained from 15 cases of glioblastoma and 34 cases of meningioma. Analysis was carried out by flow cytometry (FCM) using suspension of single separated cells stained with propidium iodide as DNA intercalating fluorochrome. Cell kinetics and proliferative activities in the different parts of a case with glioma and a case with meningioma were studied by FCM and for morphological changes (LM).
From the distribution of their DNA content the 14 specimens of glioblastoma were classified into 3 types. Egde of the gliobastoma manifested 2 peaks in the DNA histograms which represented the highest increase in the proportion of G2+M phase and S phase. Periphery of the glioblastoma revealed the infiltrative tumor cells by FCM and LM. However, the DNA histogram of the meningioma showed the same patterns in the different regions.
FCM represented the clinical diagnostic value of the malignancy of the brain tumor and will be applied to study the proliferative activities of tumor cells.
