Abstract
It is recently said that enzyme immunoassay (ETA), one of the non-isotopic immuno-assay, is almost equal to radioimmunoassay (RIA) about accuracy and sensitivity, and ETA is utilized in clinical laboratories.
In the present paper basic studies were perfomed to measure serum Ig E by EIA kit (Phadezym Ig E PRIST). The measurement theory is sandwich method similar to RIA method, and the measurement was done under explanation attached to the kit. In every 10 samples of 3 different concentrations, reproducibility was investigated and the coefficient of variation (CV) were 5.8% (x=39.8IU/ml), 18% (x=458.5IU/ml) and 19% (x=2030IU/ml), respectively. CV of reproducibility of each day (n=5) were 8.9% (x=23.4IU/ml), 12.4% (x=326IU/ml) and 13.6% (x=1530IU/ml), respectively. In samples of high Ig E level accuracy was slightly low, so in such samples determination of dilution rate will be difficult points. But it will be within permissible limits for routine examination, and this method will be useful for measuring serum Ig E level in the moderate or small-size laboratories. Development of more accurate method for high concentration of Ig E will be expected
