Abstract
In order to investigate time course of effects of chemotherapy and radiotherapy on pancreatic cancer, we used the monolayer culture of a human pancreatic cancer cell line, SUIT-2 which produces CEA and CA19-9. Into the culture various kinds of anti-cancer drugs (MMC, ADR, FAR, 5FU, FT, CDDP, CPT-11) were administered for a period of 2 days, followed by irradiation with 60Co. Observation period was 20 days, during which we measured LDH, CEA and CA19-9 in medium, DNA, protein, CEA and CA19-9 in cells in addition to observation of cells through microscope. Effects of anti-cancer drugs on pancreatic cancer cells were more greater than those of irradiation when doses of anticancer drugs were enough. By using a lot of anti-cancer drugs, LDH value in medium increased at early time and decreased later. CEA and CA19-9 values in medium decreased with the lapse of time, while they were increased later when small doses of the drugs had been administered. DNA, protein, CEA and CA19-9 values in cells showed a decrease in accordance with increased doses of the drugs. Effects of ET and CPT-11 were marginal compared with the other drugs.
