Abstract
Using the human peripheral blood normal neutrophils and the promyelocytic leukemia cell line (HL-60), the antigen of anti-neutrophil antibodies (ANA) in patients with copper deficiency was investigated. These patients were classified as group A and group B; Group A was ANA positive with neutropenia while group B was ANA positive without neutropenia. 1) The serum containing ANA from group A showed low binding (0-6.1%) to HL-60 cells, but that from group B bound to HL-60 (64.2-87%). 2) Induced differentiation of HL-60 by DMSO enhanced expression of CD 16 and CD 11b. The binding of ANA from group A patients to differentiated HL-60 was increasd (10.0-14.6%). 3) The monoclonal antibody of CD 16 showed the inhibition of ANA binding of group A to the peripheral neutrophils, but not that of group B. These data suggested that ANA in patients with copper deficiency and neutropenia were recognizing Fcγ receptor III as antigen.
