Abstract
We studied the allergenicty of non-steroidal antiinflammatory drugs (NSAIDs) in delayed-type hypersensitivity (DTH) by leukocyte migration test (LMT) in order to determine the causative drug in a patient displaying hypersensitivity to NSAIDs, An LMT was preformed using an indirect agarose-plate method. The concentration of tested antigen drugs were their therapeutic concentration level. Lymphocytes collected from the peripheral blood of patient were adjusted to a cell count of 1.25×106 cells/ml and incubated with the tested drugs at 37°C for three days in CO2-incubator. The supernatant was isolated, and the migration test was performed as follows. Granulocytes collected from healthy humans were adjusted to a density 2.5×105 cells/μl, suspended in the supernatant fluid which had been isolated as described above, and migrated on an agarose plate for 48 hours. Migration index (M. I.) values were calculated from the area of the field of migration. The leukocyte migration activatory factor (LMAF) was detected in tested 5 cases NSAID sensitive patients. On other hand leukocyte migration inhibitory factor (LMIF) was not detected. Our findings indicate that tiaramid hydrochloride and diclofenac Na induce LMAF production by lymphocytes and thereby induce their NSAID drug hypersensitivity. Also these findings indicate that LMT is valuable in detecting the causative drugs in NSAIDs hypersensitive patient, and DTH playss a major role in the pathogenesis of this hypersensitivity.
