Abstract
The modification method of Selivanoff's reported by Dr Ando in 1950 was applied to 139 cases of 4th to 10th month of pregnancy
Reagent (A) concentrated HC6 and, H2O 4 were mixed and filtered.
Reagent (B) 2% Resorcin solution.
Equal amount of ether and urine are mixed, shaken and is filtered. For several minutes it is immersed in water bath (40°-45°C) and kept warm until no foames produced by shaking. It is kept in room temperature.°CTechnique: a drop of urine treated by ether is taken in the tublet (ca 7cm length., and ca 6cm diameter). 15 drops of reagent (A) and a drop of reagent (B) are added to it and warmed up in the boiling hot water for one minute.
Determination: Light red or pink is SRM (+) which indicates female. Light yellow is SRM (-) which indicates male.
The results of 139 cases which were examined by above method and which became evident after delivery were as follows:
1) The positive rate was 95.4%≥P(+)≥83.1% in male and 83.2%≥P(-)≥67.9% in female with the level of significance in 5%
2) As the reactive substance of this reaction seems to decrease or disappear gradually as the term is approached. Therefore the test should be repeated several times after the 7th or 8th month of pregnancy.
3) A fresh urine; at least within 30 minutes after urination should be used for examination. The positive subetance seems to disappear with the lapse of time.
4) It must be noted that the reaction of the urine may become positive if quinine is given.
5) The result in the case of twin is uncertain.
