Abstract
Numbers of studies on the morphology and the mode of growth of TB bacilli have been reported. For the cytological investigations of human type TB bacilli by means of electronmicroscope, the present authors designed a slide-collcdion-culture method, resulted from the investigation on the technique of preparing samples by slide-culture method. By this method, the process is relatively simple, the influences from outside is restricted and the changes of cell structures and the modes of multiplication can be successively observed under electron micrescope as well as through ordinary microscopes. Samples of TB bacilli were treated with Anderson's extraction method or with the method of removing the nucleic acid.
The results obtained were as follows:
1) Through alcohol-ether treatment, non-transparent bodies (body-A) increased slightly their transparency and the RNA of body-A was depolimerized. The cells were condensed, still the distribution of the RNA could be observed.
2) Through chloroform treatment, preceded by alcohol-ether extraction treatment the contour of body-A became indistinct and the cells increased their density.
3) Through chloric acid-alcohol-ether treatment, preceded by the successive employment of alcohol ether and chloroform extraction treatment, the body A could not generally be observed and the Feulgen-positive granules disappeared. The contour of cells body as not clear-cut and the cells had increased density.
4) Through any method of trichloric acid treatment, body-A disappeared and almost all the granules became undetectable by means of whatever staining methods employed. The Feulgen's reaction turned to negative, and the cells lost their nature of affinity to pyronin.
