Chromosome Botany
Online ISSN : 1881-8285
Print ISSN : 1881-5936
ISSN-L : 1881-5936
Somatic chromosome differentiation in three species of the Byblis liniflora complex (Byblidaceae)
Kenji FukushimaKatsuya NaganoYoshikazu Hoshi
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2008 Volume 3 Issue 3+4 Pages 95-99


We conducted chromosomal characterizations of three species in the Byblis liniflora complex using sequential fluorescent staining with chromomycin A3 (CMA) and 4',6-diamidino-2-phenylindole (DAPI), and fluorescence in situ hybridization method. Byblis filifolia and B. rorida had the diploidal chromosome number of 2n=16, while B. liniflora had the tetraploidal chromosome number of 2n=32. All chromosomes of three species were median-centromeric. The primary constrictions, which contain centromeric regions, showed slightly CMA-positive stainability. Two CMA-positive DAPI-negative segments were observed on two chromosomes of the diploids B. rorida and B. filifolia, and the tetraploid B. liniflora. The 45S rDNA signals were detected on two chromosomes of the diploid and the tetraploid species, respectively. The positions of the 45S rDNA were corresponded to CMA-positive DAPI-negative segments.

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© 2008 International Society of Chromosome Botany
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