A molecular genetics of Drosera spatulata complex by using of RAPD analysis

Abstract

RAPD analysis was carried out using to infer the characteristics of RAPD generated DNA fragment and to investigate phylogenetic relationships in three species of D. spatulata complex with different types of genome compositions and ploidy levels. A total of 60 decamer random sequences were initially screened to select using analysis primers. The result of RAPD analysis in the present study showed that D. rotundifolia and D. tokaiensis clustered more closely than D. spatulata. Thus, DNA fragments obtained from RAPD primer might have a priority to amplify certain DNA from the haploid genome set with larger chromosome size than that of smaller size in Drosera spatulata complex species.