Abstract
Hybridity, genome differentiation and relationship were investigated in an interspecific F1 hybrid (2n=45) artificially produced between Chrysanthemum latifolium (Shih) H. Ohashi et Yonek. (formerly: Ajania latifolia Shih) (2n=36) and C. grandiflorum Ramat cv. ‘Red Betty’ (2n=54) by FISH (fluorescent in situ hybridization) and GISH (genomic in situ hybridization) using 5S, 45S and total genomic DNA labeled probes. Seventy-three hybrid seedlings were successfully produced only in vitro. FISH on the chromosomes of the hybrid had 11 signals of 45S rDNA sites, while five and six signals of the 5S rDNA were detected. GISH distinguished approximately 27-yellow-colored chromosomes were hybridized with the probe of C. latifolium and 18 redcolored were not, which could be characteristics of C. grandiflorum cv. ‘Red Betty’. Nine out of the 27 chromosomes were common homologous chromosomes of the two genomes. The 18 red-colored chromosomes, which isolated by GISH without using blocking DNA, might come from a distantly related ancestor of C. latifolium.
