Newly naturally appeared Pogonia isolated from Pogonia japonica and P. minor in Japan analyzed by RAPD (Randomly Amplified Polymorphic DNA)

Abstract

Two new forms of Pogonia different from P. japonica and P. minor were found in Japan: One was called ‘Miyamatokisou’ collected in Mt.Hakusan and the other one was still unnamed and collected in Higashi-Hiroshima City, Hiroshima Prefecture. The karyotype of Pogonia called ‘Miyamatokisou’ was 2n=18 and consisted of 18 median-centromeric chromosomes but was a little bit different from that of P. minor (2n=18). That of Pogonia collected Higashi-Hiroshima-City was 2n=20 that was consisted of 16 median-centromeric chromosomes and four terminal-centromeric chtomosomes, and similar to that of P. japonica (2n=20). Origin and relationships of those two new forms were analyzed by RAPD (the randomly amplified polymorphic DNA) technique: According to the results of RAPD obtained here, Pogonia called ‘Miyamatokisou’ and Pogonia from Higashi-Hiroshima City had different DNA fragment-patterns from P. japonica, P. minor and their F₁ hybrids. Additionally, Pogonia called‘Miyamatokisou’was different from Pogonia originated from Higashi-Hiroshima City although their flower shape was similar to each other. It was confirmed that newly naturally developed Pogonia from P. japonica and P. minor existed in nature according to the RAPD analysis.