Abstract
The members of the tribe Anthemideae, the Asteraceae distributed all over the world, could have a large synteny with common genes and the polyploid series with the basic chromosome number of X=9 (Kondo et al., 2010). The present study was made by artificial crosses among the intra- and interspecific combinations which may perform certain amplification of genic diversities of Chrysanthemum cultivars with aid of the artificial ovule culture. The artificial hybrid-plants obtained during the course of the present investigation were analyzed genomic sequences and become bridges or entrances of gene transfer to other species as an important genetic resource. The present study reported such four hybrid combinations as: (1) C. morifolium Ramat. cv. ‘Enmeiraku Pink Form’, 2n=54, hexaploid X C. zawadskii Herbich. (ID No. sp3 2004 04-KR-004-12), 2n=54. (2) C. nematolobum Hand.-Mazz., 2n=18, diploid X C. indicum L., 2n=39, (3) C. yezoense T. Mack., 2n=90, decaploid X C. zawadskii Herbich. ID No. sp3 2004 04-KR-004-12, 2n=54. (4) C. zawadskii Herbich. ID No. 07C-9-7-4, 2n=36 X C. maximowiczii Tzvelev., 2n=54. They were acclimatized in a greenhouse and analyzed as true-hybrid plants by RAPD-PCR, PCR using SGR gene and karyotypes. The karyotypes of their hybrids were compared their genetic characters. As the result, they were discriminated all 18 hybrids. They had commonly almost intermediate characters between their parents. Those ten hybrids acclimatized in a greenhouse showed variations in chromosome construction compared to those of their parents.
