Abstract
32×32 acetylcholine (ACh) image sensors using enzyme-immobilized polyion complex membrane were successfully required real time imaging of ACh distribution with better stability and improve responsibility. Nowadays, acetylcholine imaging technology has a lot of potential for human activity analysis and localization of disease. The proposed Charge-Transfer Technique ACh Image Sensor (CTTAIS) is a very unique device due to not only determine quantity but also acquire real time imaging of behavior of biological matter without label.