Three-Dimensional Motion-Picture Imaging of a Biological Object by Parallel Phase-Shifting Digital Holographic Microscopy using a High-Speed Camera

Abstract

We report three-dimensional (3D) motion-picture imaging of a biological object in the microscopic field of view by parallel phase-shifting digital holographic microscopy using a high-speed camera. Parallel phase-shifting digital holographic microscopy is a technique for high-quality and instantaneous 3D imaging of specimens in the microscopic field of view. This microscopy has been experimentally verified. This time, by applying a high-speed camera to the constructed optical system, we succeeded in recording motion-pictures of a dynamic motion of a biological object in water at 20,000 frames-per-second, which is the fastest among the previous reports on 3D imaging of a biological object.