Immunohistochemical study of localizations of basic fibroblast growth factor, fibroblast growth factor-receptor and transforming growth factor-β₁ on the periodontal tissue during experimental tooth movement.

Abstract

The purpose of this study was to confirm the localizations of basic fibroblast growth factor (bFGF), FGF-receptor (FGF-R) and transforming growth factor-β₁ (TGF-β₁) on the periodontal tissues during experimental tooth movement, by means of immunohistochemistry, using polyclonal antibodies. Male S. D. rats were used, and experimental tooth movement was performed under ether anesthesia according to the technique by Waldo. Orthodontic elastics were inserted between the left upper lst and 2nd molars. The rats were killed at 6 and 18 hours, and 3 and 7 days after the experiment. Paraffin sections 5μm in thickness cut from the left upper 1st molars and alveolar bones were stained immunohistochemically.The sections cut from the right upper molars were used for a control. On the tension side, there were immunoreactivities for bFGF and TGF-β₁ on the osteoblasts at 18 hours, 3 and 7 days. There were immunoreactivities for FGF-R on the osteoblasts at 6,18 hours. On the pressure side, there were immunoreactivities of the osteoclasts to bFGF and TGF-β₁ at 18 hours, 3 and 7days. There were immunoreactivities of the osteoclasts to FGF-R at 6,18 hours. These results indicated that bFGF, FGF-R and TGF-β₁ controlled alveolar bone resorption and formation during the experimental tooth movement.