Original Papers
High-Speed Autofocusing of Cells Using Radial Intensity Profiles Based on Depth From Diffraction (DFDi) Method
2013 Volume 3 Issue 1 Pages 13-21
Details
2013 Volume 3 Issue 1 Pages 13-21
In this paper we propose new image features named radial intensity profiles (RIP) for high-speed autofocusing of cells based on our Depth From Diffraction (DFDi) method. DFDi is a method of extracting depth information of target cells from a single defocused image containing their diffraction patterns. High-speed focusing of separated yeast cells with 20 ms response time and continuous autofocusing with a scanning microscope were demonstrated. The successful continuous autofocusing shows that image-based high-throughput measurement of cells could be realized using the proposed algorithm.
