Abstract
The sterilization ability of the chlorine dioxide（ ClO₂） gas, which has been used as an alternative decontamination agent of carcinogenic formaldehyde gas, is investigated. The validity of the sterilization condition based on a 60 m3 actual production line of clinical experimental medicine is evaluated. As the result, all of the 42 BIs （biological indicators） in the production line were negative. In addition, the fast and reliable decision protocol by a Micro Colony-ATP （Adenosine Triphosphate） method as an alternative method to the culture and measurement method（ gas measurement method, plate culture method）of BI bacteria, which took several days to several weeks for decontamination judgment is devised and validated. As the result, we confirmed that the judging decontamination effect was possible within 12 hours. The combination of chlorine dioxide gas decontamination and the Micro Colony - ATP method can further increase the convenience of users of decontaminated facilities because the decontamination judgment result can be confirmed, and the operation can be started by the next morning after the decontamination work.
