Abstract
A new thin layer chromatographic (TLC) method has been developed to identify urinary stains of human being and animals. For this experiment, three TLC plates (silicagel, cellulose and RP-18) and several developing solvents were investigated. The cellulose plate was selected as a TLC plate for good coloration and methanol—pH 9.18 boric acid buffer (3 : 1, v/v) was selected as the developing solvent for good separation.
The Rf values (and coloration) of urea, allantoin, uric acid and creatinine as urine standard substances were 0.62 (red), 0.44 (red), 0.20 (dark blue) and 0.60 (orange), respectively. Some animals (dog, cat, hamster, etc.) and human urine were identified easily by the comparison of detection of uric acid and allantoin.
On the experiment of human and dog urinary stains, urea, uric acid and creatinine were detected in human urinary stains which had been two weeks old. On the other hand, urea and allantoin were cleary detected in the dog urinary stains which were two months old. This method is a simple and rapid analysis technique for the identification of urinary stains.
