Abstract
We developed a highly sensitive and rapid ABO genotyping method based on the polymerase chain reaction (PCR) using TaqMan kit, in which three single nucleotide polymorphisms (SNPs: positions no. 261, 796 and 803) were targeted and searched with original probes. This method enabled us to confirm genotypes OO, AB (10 samples each), AO, BO, AA, and BB (1 sample each) and phenotypes A and B (10 samples each). All procedures from PCR took only 2 hours and 30 minutes. The TaqMan probe PCR method required only 1.2 ng of template DNA for ABO genotyping. It also allowed us to confirm the presence of PCR inhibitors and to estimate the quantity of template DNA by using real-time PCR, which would be utilized in subsequent examinations, as with the Multiplex STR method.
