メチレンジオキシアンフェタミン（MDA）抗体の作製とその抗体の関連化合物との反応性

田中 佐知子; 渡辺 紫織; 古家 恵子; 野村 琢磨; 貝崎 明日香; 伊藤 勝敏; 藤井 幹雄; 堀 久美子; Nastin Actor; 青木 公子; 沼澤 聡; 鳥居塚 和生; 荒川 秀俊; 伊田 喜光; 吉田 武美

doi:10.3408/jafst.13.125

Original Article

Preparation of Methylenedioxyamphetamine Antibody and Its Reactivity to the Related Compounds

Sachiko Tanaka, Shiori Watanabe, Keiko Furuya, Takuma Nomura, Asuka Kaizaki, Katsutoshi Ito, Mikio Fujii, Kumiko Hori, Nastin Actor, Kimiko Aoki, Satoshi Numazawa, Kazuo Toriizuka, Hidetoshi Arakawa, Yoshiteru Ida, Takemi Yoshida

Author information

Sachiko Tanaka
Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University
Shiori Watanabe
Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University
Keiko Furuya
Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University
Takuma Nomura
Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University
Asuka Kaizaki
Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University
Katsutoshi Ito
Department of Analytical Chemistry, School of Pharmaceutical Sciences, Showa University
Mikio Fujii
Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Showa University
Department of Organic Chemistry, School of Pharmaceutical Sciences, Toho University
Kumiko Hori
Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Showa University
Nastin Actor
Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University
Kimiko Aoki
Nihon Pharmaceutical University
Satoshi Numazawa
Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University
Kazuo Toriizuka
Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Showa University
Hidetoshi Arakawa
Department of Analytical Chemistry, School of Pharmaceutical Sciences, Showa University
Yoshiteru Ida
Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Showa University
Laboratory of Natural Product Chemistry, Yokohama College of Pharmacy
Takemi Yoshida
Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University

Keywords: Anti-methylenedioxyamphetamine antibody, Methylenedioxyamphetamine, ELISA, Methylenedioxymethamphetamine

JOURNAL FREE ACCESS

2008 Volume 13 Issue 2 Pages 125-132

DOI https://doi.org/10.3408/jafst.13.125

Details

Abstract

Structures of methylenedioxymethamphetamine (MDMA) and methylenedioxyamphetamine (MDA) are very similar to methamphetamine (MA) and amphetamine (AP), respectively. Immunoassay is one of useful methods detecting drugs. We have designed to prepare the antibody for recognizing MDMA and MDA. MDA was conjugated with bovine serum albumin (BSA) by glutaraldehyde method. Rabbits were immunized with MDA-BSA to obtain anti-MDA antibody and IgG fraction from the antiserum was used as an antibody. The cross-reactivity of the antibody was tested by an enzyme-linked immunosorbent assay (ELISA). MDMA showed higher reactivity to the antibody than MDA probably due to similar structures with methylenedioxy moiety. On the other hands, methamphetamine and amphetamine had low cross-reactivity to this antibody. The range of MDMA measurable by ELISA was 0.01-1 μg/well. MDMA in urine could be determined in the range of 0.2 to 20 μg/ml by ELISA using the antibody.

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