Abstract
This report describes a non-PCR-based ABO genotyping method using Loop-Mediated Isothermal Amplification (LAMP). LAMP method, based on isothermal DNA amplification, enables the amplification of high efficiency and within a shorter time. Three single nucleotide polymorphisms (SNPs) in the ABO gene (261, 796, and 803 np) were examined using four primer sets (O, non-O, B, and non-B). Then ABO genotypes were determined from combinations of positive reactions. All procedures from amplification to detection took only 1 hour because neither DNA extraction for amplification nor electrophoresis for allele discrimination was required. These results show that ABO genotyping using LAMP is convenient and useful for routine work to support serological typing.
