A comparison of three methods of DNA extraction from bone and tooth samples

Abstract

 DNA obtained from hard tissues, such as bones and teeth, is used to identify highly decomposed or skeletonized corpses. Effective DNA extraction is critical; the quantity and quality of DNA and the levels of co-extracted PCR inhibitors greatly influence subsequent steps. We evaluated three methods used to extract DNA from bones and teeth: use of the QIAamp DNA Mini Kit (Qiagen), the standard phenol/chloroform-based method, and the PrepFiler Express BTA Forensic DNA Extraction Kit (Thermo Fisher Scientific). We modified the protocols of the two kits. We assessed DNA quantity and quality. The QIAamp DNA Mini Kit yielded more DNA and more informative STR profiles than did the other methods; that kit is optimal.