Abstract
The reaction principle of the turbidimetric method for urine protein using a cationic detergent (benzethonium chloride) was investigated. The turbidity generation of serum albumin and γ-globulin occurred at approximately pHs 5.5 and 6.0, respectively. This reaction was reversible and dependent on pH, and the generated turbidity was eliminated by decreasing the pH by adding hydrochloric acid. The turbidity generation was considered to be caused by the binding of the positively charged cationic detergent to the negatively charged protein.
