Abstract
The genotype of the hepatitis B virus (HBV) is an important factor for treatment. Genotyping by EIA has been performed in Japan. In this study, we developed a new system using type-specific polymerase chain reaction (PCR). A total of 84 specimens (EIA genotype A, 8; genotype B, 11; genotype C, 60; genotype D, 1; not typed: 4) were analyzed using the new system. The genotype was determined in 82 of the 84 specimens (97.6%) (genotype A, 8; genotype B, 13; genotype C, 60; genotype D, 1). In addition, it was possible to analyze subgenotypes Aa/Ae and Bj/Ba by tetra-primer ARMS-PCR. Regarding the clinical utility of this new system, it has high capacity to classify the HBV genotypes and subgenotypes, which can support diagnosis.
