2015 Volume 64 Issue 1 Pages 40-47
There are reports on new parameters (NEUT-X and NEUT-Y) of neutrophils and their relationships with toxic granules in patients with inflammatory diseases. However, there is no report yet on vacuolization and on these parameter relationships. Therefore, we analyzed the relationship between the results of immunoassays and morphological changes of neutrophils in relation to NEUT-X and NEUT-Y in patients with inflammatory diseases. In this study, the patient group showed significantly higher NEUT-X and NEUT-Y levels than the control group. These levels showed a significant correlation with CRP level and PCT, but not with neutrophil count and white blood cell count. Also, toxic granules and vacuolization were reflected as cytoplasmic complexities formed by NEUT-X, and the increase in the number of immature neutrophils was reflected as an increase in the quantity of intracellular RNA induced by NEUT-Y. On the other hand, without intending to reflect bacterial infection directly, NEUT-X and NEUT-Y had captured the reactivity change of neutrophils in response to inflammation. Furthermore, in this study, the NEUT-X and NEUT-Y levels were high owing to severe sepsis. NEUT-X and NEUT-Y used as markers showed insufficient specificity and diagnostic sensitivity, but objective data can be obtained quickly and easily using an analyzer. In addition, these parameters may be helpful for diagnosing sepsis.
