Abstract
The performance of a modified selective solubilization assay for serum low-density lipoprotein cholesterol (MetaboLead® LDL-C) was compared with that of the currently used assay (Determiner® L LDL-C). The within-run coefficients of variation (CVs) for the modified LDL-C assay ranged from 0.5 to 1.3% (n = 29) for pooled sera. The range of run-to-run CVs was from 0.4 to 0.6% (n = 10). The assay results for the serially diluted LDL-C standard solution showed excellent linearity up to 677 mg/dl. No interference was observed when bilirubin, hemoglobin, Intralipid®, rheumatoid factor, heparin, or Intrafat® was included in samples for the measurement of LDL-C with the modified assay. Moreover, there was a good correlation between the results of the modified and the currently used assays (y = 0.95x − 0.9, r = 0.995). In samples with high TG levels, the LDL-C level determined by the currently used assay was higher than that determined by the modified assay. The difference in LDL-C level between the modified assay and the modified BQ method was smaller than that between the modified assay and the currently used assay. This may be due to the presence of remnant-like particle cholesterol in serum. In conclusion, the modified assay can be used to measure serum LDL-C levels, which are close to those measured by the modified BQ method.
