Abstract
In this study, we name artifacts “bamboo blinds”, which include chattering and bamboo-blind-like cracks of a section, and determine the onset mechanism and method of eliminating such bamboo blinds. As a result, in 3-um-thick sections, a few bamboo blinds broke out in a region where fibers densely aggregated, such as collagenous fibers and muscle fibers, but many bamboo blinds occurred in glandular epidermal cells. The larger the thickness of the sections, the faster the velocity of slicing, and the lower the temperature of the sample blocks, the more easily the bamboo blinds broke out. For eliminating bamboo blinds, rehydration on the surface of blocks was effective. From these findings, we consider that bamboo blinds break out because of complex factors such as weakening of tissue cells due to dehydration during the preparation of tissue blocks, existence or nonexistence of qualitative or quantitative interference from fibers and paraffin, and slicing techniques.
