2016 Volume 65 Issue 4 Pages 424-430
The whole parathyroid hormone (PTH) assay can exclusively measure bioactive 184 PTH, reflecting parathyroid function precisely without the effects of renal function. In this study, we evaluate the performance of the chemiluminescence enzyme immunoassay (CLEIA)-based whole parathyroid hormone (PTH) assay and analyzed variable factors. The performance evaluation demonstrated preferable repeatability and intermediate precision. Serum and EDTA2Na plasma samples showed good correlation, although plasma samples had superior sample storage stability. A high level of rheumatoid factor tended to be associated with the decrease in the whole PTH value. Whole PTH values were approximately 60% of intact PTH values and well correlated with intact PTH. Increased whole PTH values were confirmed after exercise, although the degree of exercise effect varied among individuals. Standing position during blood sampling tended to result in high whole PTH values compared with recumbent position. Decreased whole PTH value was confirmed in the samples obtained from whole blood left at 25 C for 4 hours or more. Whole PTH values were decreased with increasing number of sample transfers to containers. At our hospital, we used a rapid measurement of whole PTH using serum samples to enable the measurement of other parameters simultaneously and reduce the volume of blood sampling. The rapid measurement of whole PTH can precisely reflect parathyroid function and it is considered to be more useful for minimizing the effects of various factors determined in this study.
