2016 Volume 65 Issue 4 Pages 472-481
Fully automatic blood transfusion systems are useful for solving problems such as individual differences in aggregation judged and dispensing errors. Here, we report the basic performance evaluation of the ORTHO VISIONTM Analyzer (VISION), which is a fully automatic blood transfusion system. Correlations of blood type tests and irregular antibody screening tests were determined using the VISION and ORTHO AutoVue® Innova System (Innova). The effects of coexisting substances, cross-matching tests, stability of blood cell reagents, and room temperature were analyzed using VISION. The rate of concordance of blood type tests in both devices was 99%. There was only one mismatch case in the reverse test. The irregular antibody detection rate was 72% for VISION (56/78), which was superior to 64% (50/78) for Innova. The intra-assay precisions of irregular antibody screening tests were all within a grade difference. In coexisting substances tests, the contrast abnormality was detected in more than 3.7 mg/dL of hemoglobin by Ficin method, and there are cases wherein a nonspecific reaction occurred with a total globulin amount of 3,600 mg/dL or more. In the cross-matching test by the LISS-IAT method, in the case of an antibody of clinical significance, only the corresponding antigen-negative blood was matched. The red blood cell reagent was confirmed to be stable for 8 days (Dia antigen). At low room temperature influenced on incubation and centrifugation, as the result, the detection rates of the cold-reactive antibodies were increased. VISION was able to conduct a test of basic transfusion in safety, accuracy and high sensitivity.
