Basic study of urinary metanephrine and normetanephrine measurements using liquid chromatograph-tandem mass spectrometer

Abstract

We performed basic evaluation studies of urinary metanephrine and normetanephrine measurements using a liquid chromatograph-tandem mass spectrometer. This method showed CVs of within-run and between-day precisions of less than 4.0%, and the dilution linearity was also good. No matrix effect was also detected. In addition, the correlation with high-performance liquid chromatograph for postcolumn derivatization and LC-MS/MS method was good (metanephrine: y = 1.000x + 0.004, r = 0.994, n = 220; normetanephrine: y = 1.014x + 0.017, r = 0.992, n = 220). This study revealed that this method using a liquid chromatograph-tandem mass spectrometer is acceptable in routine tests.