Basic study of propafenone and 5-hydroxypropafenone measurements in serum using a liquid chromatograph-tandem mass spectrometer

Abstract

We performed basic evaluation studies of propafenone and 5-hydroxypropafenone measurements in serum using a liquid chromatograph-tandem mass spectrometer (new method). Results obtained by this method showed CVs of within-run and between-day precisions of less than 3.0%, and the accuracy was less than 13%. The dilution linearity was also good. No matrix effect was also detected. In addition, the correlation between the conventional and new methods was good (propafenone: y = 1.018x - 3.852, r = 0.996, n = 100; 5-hydroxypropafenone: y = 1.059x + 1.846, r = 0.997, n = 100). This study revealed that this new method is acceptable for use in routine tests.