2022 Volume 71 Issue 3 Pages 412-416
Background: Transmembrane domain containing 3 gene (Tm2d3) has been implicated in Notch signaling and development of Alzheimer disease. To investigate the functions of Tm2d3, we analyzed a knockout mouse strain with an exon of Tm2d3 deleted using the Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) 9 method. We analyzed the timeline in which the Tm2d3 knockout mice can be obtained. Methods and results: To obtain mice with knocked out Tm2d3, which is an autosomal gene, we crossed pairs of heterozygotes and analyzed the genotypes of the offspring mice by the PCR method. The genotypes of the obtained mice were only the wild type and heterozygotes, and there were no Tm2d3 knockout mice. Next, the pregnant mice obtained by the same mating method were cesarean sectioned over time to examine the fetal genotypes. Tm2d3 knockout mice were present at a rate of about 1/4 until embryonic days 9.5 to 16.5. Conclusion: Results suggest that the Tm2d3 knockout allele was a recessive lethal gene.
