Abstract
The CN-6500 fully automated blood coagulation analyzer (CN-6500; Sysmex Corporation) is an instrument that combines a measurement for coagulation-fibrinolysis molecular markers. In this study, we evaluated the basic performance of the CN-6500 in measuring thrombin-antithrombin III complex (TAT), plasmin-α2 plasmin inhibitor complex (PIC), and tissue plasminogen activator-plasminogen activator inhibitor complex (tPAI-C), which are molecular markers of the coagulation-fibrinolysis. The evaluation included repeatability, reproducibility, linearity, limit of detection, influence of interfering substances, biotin addition test, and correlation evaluation between CN-6500 and HISCL-5000 (Sysmex Corporation). The results of repeatability, reproducibility, linearity and limit of detection were favorable. No influence of hemolytic hemoglobin, bilirubin C and F, or interfering substances was observed. However, the three subject items were affected by biotin as a relative value of −10% or more. The correlation between the instruments (HISCL-5000: x, CN-6500: y) was good for TAT (y = 1.013x − 0.269, r = 0.998), PIC (y = 1.026x − 0.134, r = 0.998) and tPAI-C (y = 0.944x + 0.028, r = 0.999). Although the biotin effect was partially observed, the CN-6500 is considered to be a useful analyser for in-hospital testing of molecular markers of the coagulation-fibrinolysis.
