On mice's stress from the homeostatic point of view and the role of oxidizing and reducing substances- (2) -

Abstract

Problem ; In order to make the stress clear, we studied here the responding process when the stimuli of Adrenalin and Acetylcholin were used, introducing the knowledge of oxidizing and reducing substances of SH and coenzym through G-SH, and taking the homeostasis as a principle.
Procedure ; Revolving wheel. Animals : 162 NA 2 mice, _??_ 1012 g. Stimuli : oxidizing and reducing substances (Vitamin C, G-SH, Fe²⁺), coenzym (Vitamin B₁, Taurin, Hypo) : 10 mg/cc 0.1 cc respectively.
The mice's momentum for 15 minutes after injection of oxidizing and reducing substances or coenzyms was recorded by means of the polygraph.
Responding processes under the condition of (1) repeating stimulation (twice stimulation) or inverse stimulation 10 minutes after Adrenalin or Acetylcholin injection ; (2) that of 20 minutes after Adrenalin or Acetylcholin injection, each under coenzym supplement ; (3) double stimulation of oxidizing and reducing substances and coenzyms ; and (4) systematic stimulation of oxidizing and reducing substances, were investigated.
Results;
1) Under repeating stimulation and inverse stimulation 10 minutes after stimulation, Vitamin B₁, and Taurin took part in the stress and the adapting process; and under that of 20 minutes after stimulation, Taurin and Hypo took part in. (Fig. 1)
2) The relations between oxidizing and reducing substances and coenzyms under the stress were as follows : Vitamin ↑ V. B₁, Taurin C→Fe²⁺→G-SH, in repeating and inverse stimulation 10 minutes after stimulation, Vitamin ↑ V. B₁, Taurin, Hypo C→Fe²⁺→G-SH in repeating stimulation 20 minutes after S., and G-SH→Vitamin ↑ V. B₁. Taurin. Hypo Vitamin C→Fe²⁺ in inverse stimulation 20 minutes after S. (Fig. 2)
3) Under the double stimulation of oxidizing substances and coenzyms, the adapting response was easy when Vitamin C or G-SH was combined with Vitamin B₁, and when Taurin and Fe²⁺ were united with Hypo. (Fig. 3)