Abstract
We have been developing many kinds of headspace（HS）sampling techniques （Aeroscent Technology®）toward reconstructing natural aromas as if we really feel them. β-damascone（1-（2,6,6-trimethyl-1,3-cyclohen-1-y1）-2-buten-1-one）and β-damascenone （1-（2,6,6-trimethyl-1,3-cyclohexadien-1-y1）-2-buten-1-one） are the key rose- odor compounds and have extremely low-odor threshold values. Although the detection of these compounds from rose essential oils has previously been reported by many researchers, the odorants have not been directly detected from living rose fowers. Thus, we attempted to detect these compounds from living roses （Rosa damascena Trigintipetala）using improved HS sampling techniques based on two typical HS analyses（Tenax adsorption tube method and Stir Bar Sorptive Extraction method）. As a result, both β-damascone and β-damascenone were detected only by the improved Tenax tube method under the optimized conditions ; 1）at the most fragrant time of rosy scent by sensory evaluation （late morning, about 10 a.m.）2）. using Tenax 2000 mg tube（10 times volume as much as normal tube）and 3）using a heat fractionation operation（150°C,5min）This method is very effective for understanding the relationship between sensory evaluation and aroma components of rose fragrance.
