Cell Survival Rate using Three-dimensional Culture Technique with Type I Collagen on Three Commercially Pit and Fissure Sealants

Abstract

Currently, pit and fissure sealants are frequently used clinically to prevent dental caries. These pit and fisher sealants are commercially available in Japan after their safety has been evaluated in various biological tests. In three-dimensional culture using type I collagen, cultured cells are contained three-dimensionally within a collagen gel, just like in living tissue. Therefore, it is possible that the results obtained are different from those of cell viability tests using two-dimensional culture. In this study, we compared cell survival rates using the MTT method using two commercially available resin-based and one glass ionomer-based pit and fissure sealants. As a result, for both types of resin-based pit and fissure sealants, a large difference in cell survival rate was observed depending on the light irradiation time, and a low cell survival rate was observed when the light irradiation time was insufficient. Furthermore, the cell survival rate of the glass ionomer-based pit and fissure sealant was slightly lower than that of the resin-based pit and fissure sealant when irradiated with light for 10 seconds. Furthermore, no significant difference was observed between 3 minutes and 5 minutes after mixing. These results reconfirm that when using pit and fissure sealants, it is necessary to pay close attention to the light irradiation time.