Attachment of Human Gingival Fibroblasts onto collagen PLA porous scaffold

Abstract

Recently, degradable materials such as poly lactic acid (PLA) were used as Guided Tissue Regeneration (GTR) membranes and/or Guided bone Regeneration(GBR) membranes in dentistry. However, these membranes did not have any biological function such as the facilitation of cell adhesion. Additionally, they do not have a 3-dimensional porous structure to arrange the cell into the part of the organization loss. In this study, PLA 3-dimensional porous scaffold was fabricated by combination of porogen method and freeze-dry method. PLA porous scaffold surface was hydrolyzed with alkaline solution and collagen which was cell-adhesive protein was immobilized on PLA porous scaffold surface using water-soluble carbodiimide. The initial attachment of human gingival fibroblasts was examined. PLA porous scaffold with collagen immobilized after 1 hr alkaline hydrolysis showed significantly higher amount of initial cell attachment than PLA porous scaffold without collagen immobilization.