Cell Viability of Mouse Cells by Exposure to the Dental Adhesives using Cell Recovery Test with 3D Culture

Abstract

To examine the cytotoxicities of 11 commercially available dental adhesive products, mouse Balb/c 3T3 cells or cells derived from mouse uterine tissue origin were three-dimensionally cultured on a type I collagen gel to conduct a cell recovery test. The products showed significantly different cytotoxicities in both cells. The results demonstrated that 4-META products tend to be more cytotoxic than those containing Bis-GMA as the main ingredient, although no significant difference was noted between the cells. However, the manufacturer had not published the amount of each composition. Therefore, we could not fully understand the relationship between the composition and cell viability. All the products allowed cell recovery without any problem.