Abstract
Dental adhesive monomers are extensively used in clinical dentistry and are indispensable for repairing hard tissue. Products commercially available in Japan have passed a number of biological safety evaluation tests, including a cytotoxicity test, and are included as dentin adhesives in managed-care equipment. However, these embryotoxicity risks of each dental adhesive are unknown. Thus, an in vitro embryotoxicity test was conducted using the Embryo Stem Test (EST). As a result, all products were within the range of "non-embryotoxicity", while some products were near "weak embryotoxicity". The products containing Bis-GMA tended to show lower IC50 and ID50 values, with some exceptions. In contrast, those containing MMA compositions tended to have few effects on cell differentiation. These dental adhesive should be investigated further regarding their biological properties.
