Attachment of Mouse Osteoblast-like Cells onto Fibronectin- or Albumin-immobilized Poly (Lactic Acid-ε-Caprolactone) Films

Megumi FUSE; Tohru HAYAKAWA; Tomomi HASHIZUME-TAKIZAWA; Hidenori SUZUKI; Hiroaki WAKAI; Makoto NAKANISHI; Yoshimine NIKI; Hong SUNG-MOON; Katsuhiro ASAKA; Tomoko KURITA-OCHIAI; Masahiko FUKUMOTO

doi:10.11223/jarde.15.3

ORIGINAL ARTICLES

Attachment of Mouse Osteoblast-like Cells onto Fibronectin- or Albumin-immobilized Poly (Lactic Acid-ε-Caprolactone) Films

Megumi FUSE , Tohru HAYAKAWA, Tomomi HASHIZUME-TAKIZAWA, Hidenori SUZUKI, Hiroaki WAKAI, Makoto NAKANISHI, Yoshimine NIKI, Hong SUNG-MOON, Katsuhiro ASAKA, Tomoko KURITA-OCHIAI, Masahiko FUKUMOTO

Author information

Megumi FUSE Corresponding author
Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan
Tohru HAYAKAWA
Department of Dental Engineering, Tsurumi University School of Dental Medicine, Yokohama, Japan
Tomomi HASHIZUME-TAKIZAWA
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba, Japan
Hidenori SUZUKI
Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan
Hiroaki WAKAI
Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan
Makoto NAKANISHI
Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan
Yoshimine NIKI
Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan
Hong SUNG-MOON
Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan
Katsuhiro ASAKA
Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan
Tomoko KURITA-OCHIAI
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba, Japan
Masahiko FUKUMOTO
Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan

Keywords: Poly (Lactic Acid-ε-Caprolactone) film, Fibronectin, Albumin, Osteoblast-like cell, Alkali treatment

JOURNAL FREE ACCESS

2017 Volume 15 Issue 1 Pages 3-10

DOI https://doi.org/10.11223/jarde.15.3

Details

Abstract

In this study, two different proteins, fibronectin (FN) or albumin (Alb), were immobilized onto poly (lactic acid-ε-caprolactone) (PLCL) films. FN is a cell adhesion protein, whereas Alb is a cell adhesion-inhibiting protein.The amount of immobilized FN on the PLCL film was significantly greater than that for Alb (p < 0.05). The number of MC3T3-E1 cells that adhered to FN-PLCL films was larger than that for Alb-PLCL films (p < 0.05) in a 90-min cell assay. After 3 days of culture, cells appeared more flattened on FN-PLCL films, and small lamellipodia and filopodia were observed. These results indicate that FN and Alb-immobilized onto PLCL films retained their cell adhesion-promoting and cell adhesion-inhibiting properties, respectively.

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