2017 Volume 15 Issue 2 Pages 85-94
Mesenchymal stem cells (MSCs) have limited capacity to proliferate in vitro, making it very difficult to acquire sufficient cell numbers for implantation. The objective of this study was to induce MSC-like cells (MSLCs) derived from human gingival induced pluripotent stem (HG-iPSCs) into osteoblast-like cells. We derived MSLCs from HG-iPSCs under feeder-free conditions. MSLCs expressing MSC-specific markers treated with osteoblast differentiation factor expressed osteoblast-specific mRNA and protein level when cultured with or without bone morphogenetic protein (BMP). BMP-2 had a positive effect on the expression of early indicators of osteogenic differentiation. However, the medium without BMP-2 promoted calcium deposition, which occurs at the terminal stage of osteogenic differentiation. MSLCs are likely to have higher proliferative capacity, and hence, in vitro-differentiated osteoblast-like cells and appropriate scaffolds can be used for bone tissue engineering.
