Study of Differentiation Level by Three-dimensional Culture of Mouse ES Cells on Titanium Alloy Powder

Koichi IMAI; Tsubasa SHIRAI; Mari AKIYAMA; Masayuki ZENNYU; Takamitsu YOSHIDA; Tomoharu OKAMURA; Muneyasu SHIDA; Hideo SHIMIZU; Shosuke MORITA; Kazuya TOMINAGA; Kazuya MASUNO; Hidenori MATSUMOTO; Tetsunari NISHIKAWA

doi:10.11223/jarde.15.159

ORIGINAL ARTICLES

Study of Differentiation Level by Three-dimensional Culture of Mouse ES Cells on Titanium Alloy Powder

Koichi IMAI , Tsubasa SHIRAI, Mari AKIYAMA, Masayuki ZENNYU, Takamitsu YOSHIDA, Tomoharu OKAMURA, Muneyasu SHIDA, Hideo SHIMIZU, Shosuke MORITA, Kazuya TOMINAGA, Kazuya MASUNO, Hidenori MATSUMOTO, Tetsunari NISHIKAWA

Author information

Koichi IMAI Corresponding author
Department of Biomaterials,
Tsubasa SHIRAI
Department of Biomaterials,
Mari AKIYAMA
Department of Biomaterials,
Masayuki ZENNYU
Department of Biomaterials,
Takamitsu YOSHIDA
Department of Biomaterials,
Tomoharu OKAMURA
Department of Oral Pathology,
Muneyasu SHIDA
Department of Endodontics,
Hideo SHIMIZU
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan
Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Osaka, Japan
Shosuke MORITA
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan
Kazuya TOMINAGA
Department of Oral Pathology,
Kazuya MASUNO
Department of Innovations in Dental Education, Osaka Dental University, Osaka, Japan
Hidenori MATSUMOTO
Department of Innovations in Dental Education, Osaka Dental University, Osaka, Japan
Tetsunari NISHIKAWA
Department of Innovations in Dental Education, Osaka Dental University, Osaka, Japan

Keywords: Ti-6Al-4V, titanium alloy, cell differentiation, ES cell, cytotoxicity

JOURNAL FREE ACCESS

2018 Volume 15 Issue 3 Pages 159-164

DOI https://doi.org/10.11223/jarde.15.159

Details

Abstract

Pure titanium surface is strongly bound to the bone when embedded as a dental implant, with high biocompatibility to the soft tissue around the implant. However, the mechanical strength of Ti-6Al-4V alloy is superior to that of pure titanium. Titanium alloy products account for about 40% of all implant products in Japan. Of the composition elements of Ti-6Al-4V alloy, vanadium has a higher toxicity than the others. Ti-6Al-4V alloys are employed in various titanium alloy implant products. In the present study, cell differentiation and proliferation levels were examined with a polished Ti-6Al-4V alloy in three-dimensional ES cell culture on collagen, demonstrating no developmental toxicity of Ti-6Al-4V alloy. However, Ti-6Al-4V alloy powder corroded with a hydrofluoric acid solution had effects on the differentiation and proliferation of ES cells.

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