Influence of Phototoxicity on 8-MOP by Human Keratinocyte NHEK Cells

Hiroki ISHIKAWA; Rie IWAI; Kaname TSUJI; Yuki MATSUSHIMA; Aoi FUKUDA; Koji YAMADA; Kazuhiro MATSUMOTO; Hiroaki YOSHIDA; Tomio ISEKI; Hideo SHIMIZU; Mamoru UEDA; Koichi IMAI; Shosuke MORITA

doi:10.11223/jarde.15.171

ORIGINAL ARTICLES

Influence of Phototoxicity on 8-MOP by Human Keratinocyte NHEK Cells

Hiroki ISHIKAWA , Rie IWAI, Kaname TSUJI, Yuki MATSUSHIMA, Aoi FUKUDA, Koji YAMADA, Kazuhiro MATSUMOTO, Hiroaki YOSHIDA, Tomio ISEKI, Hideo SHIMIZU, Mamoru UEDA, Koichi IMAI, Shosuke MORITA

Author information

Hiroki ISHIKAWA Corresponding author
Graduate School of Dentistry (First Department of Oral Maxillofacial Surgery),
Rie IWAI
Department of Oral Maxillofacial Surgery,
Kaname TSUJI
Department of Oral Maxillofacial Surgery,
Yuki MATSUSHIMA
Department of Oral Maxillofacial Surgery,
Aoi FUKUDA
Department of Oral Maxillofacial Surgery,
Koji YAMADA
Department of Oral Maxillofacial Surgery,
Kazuhiro MATSUMOTO
Department of Oral Maxillofacial Surgery,
Hiroaki YOSHIDA
Department of Oral Maxillofacial Surgery,
Tomio ISEKI
Department of Oral Maxillofacial Surgery,
Hideo SHIMIZU
Department of Oral Maxillofacial Surgery,
Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Osaka, Japan
Mamoru UEDA
Graduate School of Dentistry (First Department of Oral Maxillofacial Surgery),
Koichi IMAI
Department of Biomaterials, Osaka Dental University, Osaka, Japan
Shosuke MORITA
Department of Oral Maxillofacial Surgery,

Keywords: phototoxicity, 8-MOP, human keratinocyte, NHEK

JOURNAL FREE ACCESS

2018 Volume 15 Issue 3 Pages 171-176

DOI https://doi.org/10.11223/jarde.15.171

Details

Abstract

Phototoxicity, i.e., cytotoxicity caused by light irradiation, is due to the biological effects of active oxygen and free radicals, generated by energy released upon the excitement of chemical substances by ultraviolet irradiation, and those of photoexcited chemical substances. We have already conducted a phototoxicity test on 8-MOP using six cell types, including mouse-derived 3T3 cells and human oral cavity-derived tumor cells, demonstrating potent phototoxicity in oral cancer-derived cells.

Since hyperkeratosis occurs in some oral mucosal diseases as in inflammatory keratosis (psoriasis), a phototoxicity test was conducted with human keratinocytes. As a result, the phototoxicity of 8-MOP was demonstrated in two-dimensional culture, and was more significant in three-dimensional culture.

Corresponding author

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