Effects of Three-day Fibroblast Growth Factors-2 (FGF-2) Supplementation on Proliferation and Osteogenic Differentiation of Cultured Adipose-derived and Bone Marrow-derived Stromal Cells

Abstract

Basic fibroblast growth factor (FGF-2) could promote proliferation of bone marrow derived stromal cells (BMSCs) and adipose derived-stromal cells (ASCs) in vitro. FGF-2 was reported to have contrasting effects on osteogenic differentiation of ASCs/BMSCs by inhibiting osteogenesis related gene expression in vitro. In this study, we examined and evaluated effects of FGF-2 on cell proliferation, osteogenic relative gene expression, and mineralization of mouse ASC/BMSC culture at different time-points. The osteogenic potential of FGF-2 on cultured BMSCs and ASCs when the growth factor was present in the culture medium for 3 days at different time points or for 7 days was different. The results of this study indicated that FGF-2 supplementation in the culture medium at as late as day 3 resulted in more mineralization in BMSCs, but a lesser effect of FGF-2 on osteoblastic differentiation of ASCs was noted.