Comparison of the Cell Differentiation Level of Mouse iPS Cells and ES-D3 Cells Using the Embryonic Stem Cell Test (EST) Protocol Attempting to Develop a New in Vitro Embryotoxicity Test

Koichi IMAI; Kazuhiko SUESE; Shinji KUSAKAWA; Akito TANOUE; Makiko KUWAGATA; Mika SENUMA; Mami FURUYA; Hiromasa TAKASHIMA

doi:10.11223/jarde.7.38

ORIGINAL ARTICLE

Comparison of the Cell Differentiation Level of Mouse iPS Cells and ES-D3 Cells Using the Embryonic Stem Cell Test (EST) Protocol Attempting to Develop a New in Vitro Embryotoxicity Test

Koichi IMAI, Kazuhiko SUESE, Shinji KUSAKAWA, Akito TANOUE, Makiko KUWAGATA, Mika SENUMA, Mami FURUYA, Hiromasa TAKASHIMA

Author information

Koichi IMAI
Department of Biomaterials, Osaka Dental University, Osaka, Japan
Kazuhiko SUESE
Osaka Dental University. School of Dental Technician and Hygienist, Osaka, Japan
Shinji KUSAKAWA
Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
Akito TANOUE
Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
Makiko KUWAGATA
Laboratory of Toxicology, Hatano Research Institute, FDSC, Kanagawa, Japan
Mika SENUMA
Laboratory of Toxicology, Hatano Research Institute, FDSC, Kanagawa, Japan
Mami FURUYA
Laboratory of Toxicology, Hatano Research Institute, FDSC, Kanagawa, Japan
Hiromasa TAKASHIMA
Laboratory of Toxicology, Hatano Research Institute, FDSC, Kanagawa, Japan

Keywords: iPS, ES-D3, embryotoxicity, EST, differentiation

JOURNAL FREE ACCESS

2009 Volume 7 Issue 1 Pages 38-43

DOI https://doi.org/10.11223/jarde.7.38

Details

Abstract

Induced pluripotent stem (iPS) cells have a developmental potential similar to that of ES cells. There is a possibility to be able to use the mouse iPS cells as in vitro embryotoxicity test according to the cell differentiation level. We examined the possibility that the mouse iPS cells could be used instead of ES-D3 cells with the Embryonic Stem Cell Test (EST). The differentiation level of the mouse iPS cells in the same protocol as the EST was tested.
A pulse was observed in ES-D3 cells at a rate of 82%. However, only 52% of iPS cells showed a pulse, with a low rate of contraction. There was a marked difference in iPS cells at the beginning of cardiac muscle contraction. The iPS cell is expected to be applied to embryotoxicity, as well as ES cells. However, research on iPS cells began only three years ago, and optimal differentiation techniques for iPS cells must be developed in near future.

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