Journal of Oral Tissue Engineering
Online ISSN : 1880-0823
Print ISSN : 1348-9623
ISSN-L : 1348-9623
ORIGINAL ARTICLE
Development of in Vitro Embryotoxicity Testing by Differentiation of Mouse ES cells using Glass Fiber
Koichi IMAIKazuhiko SUESEAkito TANOUEKazuaki NAKAMURAHiromasa TAKASHIMA
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JOURNAL FREE ACCESS

2011 Volume 9 Issue 2 Pages 81-87

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Abstract

We have proposed the embryotoxicity testing of mouse ES cells embedded in a collagen gel matrix. The rate of differentiation of ES-D3 cells into cardiomyocytes was measured using a glass fiber as a scaffold material for collagen gel. In order to design a test method for embryotoxicity under an exposure condition where broader applications would be possible in various fields using a glass fiber as a non-animal-derived scaffold material. We measured as the test materials using TEGDMA, EGDMA and 2HEMA. There was no significant difference found with glass fiber and collagen gel matrix. Therefore, it was suggested that glass fiber could be used as the scaffold material for in vitro embryotoxicity test. Furthermore; the Embryonic Stem Cell Test may be able to apply to the environmental field etc.

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© 2011 by Japanese Association of Regenerative Dentistry
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