Abstract
It is thought that the protein crystal grows larger and gets better quality under microgravity than the crystal obtained on the ground, since there is neither convection nor sedimentation. We investigated the crystallization conditions in detail about NfsB, an oxygen-sensitive nitroreductase from Escherichia coli, and considered the crystallization mechanism by using phase diagram. This research tends to acquire the high generality knowledge in protein crystallization as research connected to elevate authenticity of the crystallization experiment under microgravity.